Mass spectrometric profiling of HLA-B44 peptidomes provides evidence for Tapasin-mediated Tryptophan editing

Mass spectrometric base composition profiling: Implications for forensic mtDNA databasing☆

In forensic genetics mitochondrial DNA (mtDNA) is usually analyzed by direct Sanger-type sequencing (STS). This method is known to be laborious and sometimes prone to human error. Alternative methods have been proposed that lead to faster results. Among these are methods that involve mass-spectrometry resulting in base composition profiles that are, by definition, less informative than the full...

P32. High resolution mass spectrometry reveals the depth and diversity of HLA-I peptidomes

T-cell responses against infected and cancer cells are initiated by recognition of HLA-I peptides (the peptidome) presented on the surface of nucleated cells. The repertoire of HLA-I peptides originates primarily from sampling the cytosolic degradation products of intracellular proteins. HLA-I peptides have been extensively studied in the last years because of their immediate use as immunothera...

HLA Class I Polymorphism and Tapasin Dependency

HLA-A2, HLA-B44 and HLA-DR15 are associated with lower risk of BK viremia.

BACKGROUND Human leucocyte antigens (HLAs) modulate immunity to polyomavirus BK (BKV). Identification of HLAs that alter the course of infection will facilitate risk stratification, and customization of pre-emptive intervention strategies. METHODS We performed a retrospective cohort study with 998 kidney transplant patients with BKV infection status confirmed by polymerase chain reaction (PCR...

Large scale mass spectrometric profiling of peptides eluted from HLA molecules reveals N-terminal-extended peptide motifs.

The majority of >2000 HLA class I molecules can be clustered according to overlapping peptide binding specificities or motifs recognized by CD8(+) T cells. HLA class I motifs are classified based on the specificity of residues located in the P2 and the C-terminal positions of the peptide. However, it has been suggested that other positions might be relevant for peptide binding to HLA class I mo...